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Ultrasound assessment of angiogenesis in a matrigel model in rats.
|Title||Ultrasound assessment of angiogenesis in a matrigel model in rats.|
|Publication Type||Journal Article|
|Year of Publication||2006|
|Authors||Stieger SM, Bloch SH, Foreman O, Wisner ER, Ferrara KW, Dayton PA|
|Journal||Ultrasound in medicine & biology|
|Date Published||2006 May|
|Keywords||Animals, Collagen, Contrast Media, Disease Models, Animal, Drug Combinations, Fibroblast Growth Factor 2, Image Processing, Computer-Assisted, Laminin, Male, Microcirculation, Neoplasms, Neovascularization, Pathologic, Proteoglycans, Rats, Rats, Sprague-Dawley, Ultrasonography, Doppler|
Matrigel, a basement membrane extract, has been extensively used in in vivo angiogenesis. Contrast ultrasound imaging (CUI) of implanted Matrigel plugs with (+bFGF) and without basic fibroblast growth factor (-bFGF) was performed 7 and 14 d after implantation, followed by histologic analysis. Statistically significant differences between +bFGF and -bFGF plugs were apparent at d 7 in both plug size and contrast enhancement (both p < 0.05). Histopathology revealed differences in microvessel density (MVD) between +bFGF and -bFGF at d 7 and d 14. A significant correlation between MVD and both power Doppler contrast-enhanced area (r = 0.65, p < 0.05) and fraction of plug enhanced (r = 0.59, p < 0.05) was present. CUI of Matrigel plugs was shown to be a robust method for distinguishing between two different angiogenic states. Ultrasound measurements of blood flow in the plugs correlated with MVD, a histologic technique used to quantify tumor angiogenesis.
|Alternate Journal||Ultrasound Med Biol|