TitleComparison of diagnostic accuracy of laparoscopic 3 mm and 5 mm cup biopsies to wedge biopsies of canine livers.
Publication TypeJournal Article
Year of Publication2018
AuthorsKimbrell, TL, Milovancev, M, Olsen, R, Löhr, CV
JournalJ Vet Intern Med
Volume32
Issue2
Pagination701-706
Date Published2018 Mar
ISSN1939-1676
KeywordsAnimals, Biopsy, Dog Diseases, Dogs, Female, Laparoscopy, Liver Diseases, Male, Prospective Studies
Abstract

BACKGROUND: Diagnostic accuracy of the 3 mm laparoscopic cup biopsy forceps for collection of tissue samples from canine livers is unproven.

HYPOTHESES/OBJECTIVES: Compare sample surface area and portal triad count between 3 mm and 5 mm laparoscopic cup biopsies and compare the histologic diagnosis obtained by each instrument to a standard necropsy wedge. The hypothesis was that more portal triads and greater sample surface area would be found with the 5 mm samples and the laparoscopic instruments would not have significantly different levels of agreement with necropsy wedge diagnosis.

ANIMALS: Twenty-one client-owned dogs undergoing necropsy.

METHODS: Prospective ex vivo study. Three samples (3 mm, 5 mm, and wedge) were taken of 2 different hepatic divisions within 24 hours of death. Morphologic diagnosis, World Small Animal Veterinary Association histologic features, surface area, and portal triad numbers were compared among the 3 samples.

RESULTS: There were significantly more portal triads (mean 21.4 versus 13.8; P < .0001) and a higher surface area (20.3 mm versus 11.5 mm ; P < .0001) in the 5 mm samples compared to 3 mm samples. Kappa coefficients and percent agreement for histologic diagnosis as compared to the wedge biopsy were not significantly different between the 2 instrument sizes (κ = 0.383 and 0.436, respectively; 67% and 69%, respectively).

CONCLUSIONS AND CLINICAL IMPORTANCE: Despite yielding smaller sample sizes, the 3 mm laparoscopic cup biopsy has a similar level of histologic diagnostic accuracy to the 5 mm instrument.

DOI10.1111/jvim.15054
Alternate JournalJ. Vet. Intern. Med.
PubMed ID29460333
PubMed Central IDPMC5866965