TitleElectrophysiological recording from parasitic nematode muscle.
Publication TypeJournal Article
Year of Publication2008
AuthorsRobertson, AP, Puttachary, S, Buxton, SK, Martin, RJ
JournalInvert Neurosci
Volume8
Issue4
Pagination167-75
Date Published2008 Dec
ISSN1439-1104
KeywordsAnimals, Electrophysiology, Ion Channels, Microelectrodes, Muscles, Nematoda, Patch-Clamp Techniques
Abstract

Infection of man and animals with parasitic nematodes is recognized as a significant global problem (McLeod in Int J Parasitol 25(11):1363-1367, 1994; Hotez et al. in N Engl J Med 357(10):1018-1027, 2007). At present control of these infections relies primarily on chemotherapy. There are a limited number of classes of anthelmintic compounds and the majority of these act on ion-channels of the parasite (Martin et al. in Parasitology 113:S137-S156, 1996). In this report, we describe electrophysiological recording techniques as applied to parasitic nematodes. The aim of this report is: (1) to promote the study of ion channels in nematodes to help further the understanding of antinematodal drug action; (2) to describe our recording equipment and experimental protocols; and (3) provide some examples of the information to be gleaned from this approach and how it can increase our understanding of these important pathogens.

DOI10.1007/s10158-008-0080-8
Alternate JournalInvert. Neurosci.
PubMed ID19005711
PubMed Central IDPMC2772203
Grant ListR01 AI047194 / AI / NIAID NIH HHS / United States
R01 AI047194-09 / AI / NIAID NIH HHS / United States
R01 AI04719406A1 / AI / NIAID NIH HHS / United States