TitleMinimally invasive synovium harvest for potential use in meniscal tissue engineering.
Publication TypeJournal Article
Year of Publication2012
AuthorsWarnock, J, Baltzer, WI, Duesterdieck-Zellmer, KF, Ott, J
JournalRes Vet Sci
Date Published2012 Dec
KeywordsAnimals, Arthroscopy, Dog Diseases, Dogs, Menisci, Tibial, Patellar Dislocation, Stifle, Synovial Membrane, Tissue and Organ Harvesting, Tissue Engineering

Tissue engineering is being investigated as a means for treating avascular meniscal injury or total meniscal loss in human and veterinary patients. The purpose of this study was to determine if an arthroscopic tissue shaver can be used to collect viable synoviocytes for in vitro culture during therapeutic stifle arthroscopy, with the long term goal of producing autologous meniscal fibrocartilage for meniscal tissue engineering. Synovium was harvested arthroscopically from 13 dogs with naturally occurring cranial cruciate ligament deficiency and obtained from 5 dogs with patellar luxation via arthrotomy. Cells harvested via arthroscopy and arthrotomy were treated with a chondrogenic growth factor protocol and analyzed for meniscal-like matrix constituents including collagens type I, II, and glycosaminoglycans. Arthrotomy and Arthroscopic origin cells formed contracted tissues containing collagen I, II and small amounts of GAG. These surgical methods provide clinically relevant access to synoviocytes for potential use in meniscal tissue engineering.

Alternate JournalRes. Vet. Sci.
PubMed ID22521160