Species: 
Bovine, Camelid
Diagnostic Section: 
Molecular Diagnostics
Test Fee: 
75.00
Setup Days: 
Monday, Tuesday, Wednesday, Thursday, Friday
Turn Around Time: 
4-5 days
Shipping Requirements: 
Label all samples. Ship overnight with cold packs in an insulated container. Ship Monday-Thursday only. Submit specimens in sealed leakproof containers.
Specimen Requirements: 

Blood: Bovids; can pool EDTA blood (up to 10 blood / pool all ages). Camelids; can pool EDTA blood (up to 5 blood / pool for > 60 days old OR up to 2 blood / pool for < 60 days) Bovine ear notches: up to 24 ear notches per pool

Collection Protocol: 

Collect ear notch (1cm/side) from each animal and place in a dry, sterile tube (e.g. serum tube-no separator gel or anticoagulant). A medium ear notch tool works well or similar tool that cuts a notch that is 1/2" wide at base. Please do not submit large pieces of ear tissue. Individual samples must be identified and submitted separately; samples will be pooled by the laboratory. Disinfect tools with 10% bleach (e.g., 100mL/3oz bleach in 900mL/27oz water) or 1% Virkon, between animals; rinse thoroughly with water. Residual disinfectant or contamination with feces or tattoo ink can yield false negative results. Do not vaccinate while collecting ear notches - this can lead to contamination with extraneous BVDV, causing false positive results. For blood specimens: ensure thorough mixing of blood in EDTA (e.g. invert tube several times immediately following collection) to prevent clotting. Clotted blood is not suitable for testing. Fill tube at least halfway to prevent PCR inhibition caused by excess EDTA.

Additional Information: 

Pooled testing for detection of bovine viral diarrhea virus (BVDV) by real time PCR. Pooled testing should not be used for animals exhibiting clinical symptoms; test is used to detect Persistently Infected (PI) asymptomatic individuals in a herd. Individual specimens within a positive pool will be identified by BVDV Ag-capture ELISA or BVDV rt-PCR for an additional fee. Please specify when specimens are submitted.