Urine - 5-10 ml (as fresh as possible to prevent degradation of the Leptospira organism and/or EDTA whole blood - 2-4 ml
Kidney (as fresh as possible to prevent degradation of the Leptospira organism)
Refrigerate urine and EDTA whole blood - Do not allow specimens to freeze.
Freeze kidney and submit each tissue individually bagged and labeled with a permanent marker.
The Leptospira spp Real-Time PCR assay can detect Leptospira species in blood during the acute phase of the infection, usually during the first week prior to seroconversion. Detection in urine is usually 7-14 days post infection, and it may or may not be detected in the blood at this time. We recommend testing both blood and urine due to variations in shedding.
**Fee is $36.75 per specimen (if submitting urine and EDTA whole blood the fee will be $73.50) for the first 3 tests. If 4 or more tests are submitted from the same herd at the same time all tests are $33.00 each.