Bovine Neonatal Diarrhea Protocol

Bovine Neonatal Diarrhea Protocol

  • All tests require that strict sample hygiene is used to prevent any cross-contamination between cases.  Samples submitted in latex gloves, OB sleeves, or other unacceptable containers will not be processed or tested.
  • Collect necessary samples using a Whirl-Pak® or Ziploc® bag for each fresh tissue collected.  DO NOT POOL TISSUES.  Clearly label bags with the name of the owner and the specimen contained within the bag.
  • Histopathology: Minimal sampling should include rumen, abomasum, duodenum, jejunum, ileum, colon and any gross lesions of the gastrointestinal tract.  Samples should be opened along the mesenteric border to expose the lumen and placed in 10% formalin.  Samples of liver, kidney, lung, spleen, and cardiac muscle should also be included in 10% formalin.
  • Molecular Diagnostics - Scours PCR Panel: Panel includes detection of Coronavirus, Rotavirus, Cryptosporidium parvum, Salmonella spp. and E. coli-K99+. Submit 3-5 grams fresh or frozen feces, or intestinal contents/scrapings in a leak proof container.
  • Virology: Ileal contents/scrapings for direct EM exam is preferred (placed in a plastic screw-top vial).  If BVDV is suspected additional fresh samples such as distal ileum, mesenteric lymph node, and thymus may be submitted for PCR and/or VI.
  • Bacteriology: Samples should include a fresh segment of colon, mesenteric lymph node, and any grossly abnormal tissue.  Aerobic culture will be performed.  Fresh samples of liver, lung, and kidney may be included if septicemia is suspected.
  • Parasitology: Fecal material should be collected in a plastic screw-top vial for subsequent Cryptosporidium exam.

NOTE: Submit all fresh tissues individually packaged in either a Whirl-Pak® or Ziploc® bag.

  • Refer to section entitled Shipping Protocols in Client Guidelines