Biological and molecular characterization of a canine hemangiosarcoma-derived cell line.

TitleBiological and molecular characterization of a canine hemangiosarcoma-derived cell line.
Publication TypeJournal Article
Year of Publication2006
AuthorsThamm DH, Dickerson EB, Akhtar N, Lewis R, Auerbach R, Helfand SC, MacEwen GE
JournalResearch in veterinary science
Date Published2006 Aug
KeywordsAngiogenic Proteins, Animals, Cell Proliferation, Dogs, Flow Cytometry, Hemangiosarcoma, Immunohistochemistry, Kidney Neoplasms, Neovascularization, Pathologic, RNA, Messenger, Tumor Cells, Cultured

Canine hemangiosarcoma (HSA) is a devastating disease. Investigation of novel therapies has been limited by the limited availability of canine HSA-derived cell lines. We report the development of a canine HSA-derived cell line, DEN-HSA, which recapitulates features of angiogenic endothelium. DEN-HSA cells were derived from a spontaneous HSA arising in the kidney of a dog. DEN-HSA displayed surface molecules distinctive of endothelial histogenesis, including factor VIII-related antigen, ICAM-1 and alpha(v)beta3 integrin. In vitro, DEN-HSA formed microvascular tube-like structures on Matrigel, and proliferated in response to a variety of angiogenic growth factors. The cells expressed mRNA and protein specific for bFGF and its receptors, and VEGF and its receptors, among others. DEN-HSA conditioned medium evoked a marked angiogenic response in a murine corneal pocket assay, indicating potent proangiogenic activity of substances secreted by this cell line. This research confirms the DEN-HSA cell line as endothelial in origin, suggests the presence of angiogenic growth factor autocrine loops, and offers the potential to utilize DEN-HSA cells for the study of novel therapies that modulate endothelial proliferation.

Alternate JournalRes. Vet. Sci.