Biological and molecular characterization of a canine hemangiosarcoma-derived cell line.

TitleBiological and molecular characterization of a canine hemangiosarcoma-derived cell line.
Publication TypeJournal Article
Year of Publication2006
AuthorsThamm DH, Dickerson EB, Akhtar N, Lewis R, Auerbach R, Helfand SC, MacEwen GE
JournalResearch in veterinary science
Volume81
Issue1
Pagination76-86
Date Published2006 Aug
ISSN0034-5288
KeywordsAngiogenic Proteins, Animals, Cell Proliferation, Dogs, Flow Cytometry, Hemangiosarcoma, Immunohistochemistry, Kidney Neoplasms, Neovascularization, Pathologic, RNA, Messenger, Tumor Cells, Cultured
Abstract

Canine hemangiosarcoma (HSA) is a devastating disease. Investigation of novel therapies has been limited by the limited availability of canine HSA-derived cell lines. We report the development of a canine HSA-derived cell line, DEN-HSA, which recapitulates features of angiogenic endothelium. DEN-HSA cells were derived from a spontaneous HSA arising in the kidney of a dog. DEN-HSA displayed surface molecules distinctive of endothelial histogenesis, including factor VIII-related antigen, ICAM-1 and alpha(v)beta3 integrin. In vitro, DEN-HSA formed microvascular tube-like structures on Matrigel, and proliferated in response to a variety of angiogenic growth factors. The cells expressed mRNA and protein specific for bFGF and its receptors, and VEGF and its receptors, among others. DEN-HSA conditioned medium evoked a marked angiogenic response in a murine corneal pocket assay, indicating potent proangiogenic activity of substances secreted by this cell line. This research confirms the DEN-HSA cell line as endothelial in origin, suggests the presence of angiogenic growth factor autocrine loops, and offers the potential to utilize DEN-HSA cells for the study of novel therapies that modulate endothelial proliferation.

Alternate JournalRes. Vet. Sci.