Detection and measurement of canine alpha-macroglobulins by enzyme immuno-assay.

TitleDetection and measurement of canine alpha-macroglobulins by enzyme immuno-assay.
Publication TypeJournal Article
Year of Publication1999
AuthorsRuaux CG, Lee RP, Atwell RB
JournalResearch in veterinary science
Volume66
Issue3
Pagination185-90
Date Published1999 Jun
ISSN0034-5288
Keywordsalpha-Macroglobulins, Animals, Dogs, Electrophoresis, Polyacrylamide Gel, Immune Sera, Immunoenzyme Techniques, Isoelectric Focusing, Male, Sheep
Abstract

The alpha-macroglobulins are broad-specificity protease inhibitors important in the regulation and clearance from circulation of biologically active proteases. Inappropriate protease activation may be a feature of canine acute pancreatitis and the ability of the animal to clear these proteases may be important in determining survival. An enzyme immunoassay for the detection and measurement of canine alpha-macroglobulins in plasma samples was developed. A reference range for the canine alpha-macroglobulins of 1.20-2.72 mg ml-1 was established from a panel of canine plasma samples, and the stability of the alpha-macroglobulins in plasma samples stored at 4 degrees C was investigated. Changes in the level of the alpha-macroglobulins during disease states involving increased endogenous protease activity can now be investigated using a rapid, repeatable and quantifiable assay.

Alternate JournalRes. Vet. Sci.