TitleBighorn sheep gut microbiomes associate with genetic and spatial structure across a metapopulation.
Publication TypeJournal Article
Year of Publication2020
AuthorsCouch, CE, Arnold, HK, Crowhurst, RS, Jolles, AE, Sharpton, TJ, Witczak, MF, Epps, CW, Beechler, B
JournalSci Rep
Volume10
Issue1
Pagination6582
Date Published2020 04 20
ISSN2045-2322
KeywordsAnimals, Bacteria, Feces, Gastrointestinal Microbiome, Mammals, Phylogeny, RNA, Ribosomal, 16S, Sheep, Bighorn
Abstract

Studies in laboratory animals demonstrate important relationships between environment, host traits, and microbiome composition. However, host-microbiome relationships in natural systems are understudied. Here, we investigate metapopulation-scale microbiome variation in a wild mammalian host, the desert bighorn sheep (Ovis canadensis nelsoni). We sought to identify over-represented microbial clades and understand how landscape variables and host traits influence microbiome composition across the host metapopulation. To address these questions, we performed 16S sequencing on fecal DNA samples from thirty-nine bighorn sheep across seven loosely connected populations in the Mojave Desert and assessed relationships between microbiome composition, environmental variation, geographic distribution, and microsatellite-derived host population structure and heterozygosity. We first used a phylogenetically-informed algorithm to identify bacterial clades conserved across the metapopulation. Members of genus Ruminococcaceae, genus Lachnospiraceae, and family Christensenellaceae R7 group were among the clades over-represented across the metapopulation, consistent with their known roles as rumen symbionts in domestic livestock. Additionally, compositional variation among hosts correlated with individual-level geographic and genetic structure, and with population-level differences in genetic heterozygosity. This study identifies microbiome community variation across a mammalian metapopulation, potentially associated with genetic and geographic population structure. Our results imply that microbiome composition may diverge in accordance with landscape-scale environmental and host population characteristics.

DOI10.1038/s41598-020-63401-0
Alternate JournalSci Rep
PubMed ID32313214
PubMed Central IDPMC7171152