TitleChlamydial development is blocked in host cells transfected with Chlamydophila caviae incA.
Publication TypeJournal Article
Year of Publication2004
AuthorsAlzhanov, D, Barnes, J, Hruby, DE, Rockey, DD
JournalBMC Microbiol
Date Published2004 Jul 01
KeywordsAnimals, Bacterial Proteins, Cell Line, Cell Line, Tumor, Chlamydia trachomatis, Chlamydophila, CHO Cells, Cricetinae, Cricetulus, Cytoplasm, Genetic Vectors, HeLa Cells, Humans, Membrane Proteins, Models, Genetic, Phosphoproteins, Transfection, Vaccinia virus

BACKGROUND: Chlamydiae produce a set of proteins, termed Inc proteins, that are localized to the inclusion membrane and exposed to the host cell cytosol. Little information exists regarding the interaction of Inc proteins with the eukaryotic cell. To examine these interactions, Vaccinia virus vectors and mammalian plasmid-based systems were used to express inc genes in mammalian cells.

RESULTS: Cells transfected with plasmids expressing Chlamydophila caviae incA were not productively infected by C. caviae. Expression of C. caviae incA also reduced inclusion formation by Chlamydia trachomatis, but not to the degree seen for C. caviae. Chlamydia trachomatis incA did not block development of either C. trachomatis or C. caviae. Deletion mutagenesis was used to demonstrate that plasmids encoding either the amino or carboxy-terminal regions of the protein, as well as the changing of a single amino acid within IncA (serine 17) could not block C. caviae infection. Immunoblot analysis of truncated IncA in a Vaccinia virus system provided evidence that serine 17 of C. caviae IncA is a target for phosphorylation.

CONCLUSIONS: These experiments provide insight into the interaction of Inc proteins with the host cell and introduce a model system where these interactions can be explored further.

Alternate JournalBMC Microbiol
PubMed ID15230981
PubMed Central IDPMC459217
Grant ListR01 AI048769 / AI / NIAID NIH HHS / United States
AI42869 / AI / NIAID NIH HHS / United States
AI48769 / AI / NIAID NIH HHS / United States