TitleControl of signaling in a MAP-kinase pathway by an RNA-binding protein.
Publication TypeJournal Article
Year of Publication2007
AuthorsPrinz, S, Aldridge, C, Ramsey, SA, R Taylor, J, Galitski, T
JournalPLoS One
Date Published2007 Feb 28
KeywordsCell Adhesion, Cell Shape, DNA-Binding Proteins, Feedback, Physiological, Gene Expression Regulation, Fungal, Genes, Fungal, MAP Kinase Signaling System, Mitogen-Activated Protein Kinase Kinases, Mitogen-Activated Protein Kinases, Phenotype, Phosphorylation, Protein Kinases, Protein Processing, Post-Translational, Repressor Proteins, RNA-Binding Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors

Signaling-protein mRNAs tend to have long untranslated regions (UTRs) containing binding sites for RNA-binding proteins regulating gene expression. Here we show that a PUF-family RNA-binding protein, Mpt5, represses the yeast MAP-kinase pathway controlling differentiation to the filamentous form. Mpt5 represses the protein levels of two pathway components, the Ste7 MAP-kinase kinase and the Tec1 transcriptional activator, and negatively regulates the kinase activity of the Kss1 MAP kinase. Moreover, Mpt5 specifically inhibits the output of the pathway in the absence of stimuli, and thereby prevents inappropriate cell differentiation. The results provide an example of what may be a genome-scale level of regulation at the interface of signaling networks and protein-RNA binding networks.

Alternate JournalPLoS One
PubMed ID17327913
PubMed Central IDPMC1803019
Grant ListP50 GM076547 / GM / NIGMS NIH HHS / United States
U54 AI054523 / AI / NIAID NIH HHS / United States
U54 AI054523R / AI / NIAID NIH HHS / United States