TitleEarly development and tissue distribution of Pseudoloma neurophilia in the zebrafish, Danio rerio.
Publication TypeJournal Article
Year of Publication2014
AuthorsSanders, JL, Peterson, TS, Kent, ML
JournalJ Eukaryot Microbiol
Date Published2014 May-Jun
KeywordsAnimals, Histocytochemistry, In Situ Hybridization, Microsporidia, RNA, Ribosomal, 18S, Spores, Protozoan, Zebrafish

The early proliferative stages of the microsporidian parasite, Pseudoloma neurophilia were visualized in larval zebrafish, Danio rerio, using histological sections with a combination of an in situ hybridization probe specific to the P. neurophilia small-subunit ribosomal RNA gene, standard hematoxylin-eosin stain, and the Luna stain to visualize spores. Beginning at 5 d post fertilization, fish were exposed to P. neurophilia and examined at 12, 24, 36, 48, 72, 96, and 120 h post exposure (hpe). At 12 hpe, intact spores in the intestinal lumen and proliferative stages developing in the epithelial cells of the anterior intestine and the pharynx and within hepatocytes were observed. Proliferative stages were visualized in the pancreas and kidney at 36-48 hpe and in the spinal cord, eye, and skeletal muscle beginning at 72 hpe. The first spore stages of P. neurophilia were observed at 96 hpe in the pharyngeal epithelium, liver, spinal cord, and skeletal muscle. The parasite was only observed in the brain of larval fish at 120 hpe. The distribution of the early stages of P. neurophilia and the lack of mature spores until 96 hpe indicates that the parasite gains access to organs distant from the initial site of entry, likely by penetrating the intestinal wall with the polar tube.

Alternate JournalJ Eukaryot Microbiol
PubMed ID24411000
PubMed Central IDPMC4008673
Grant ListP40 RR012546 / RR / NCRR NIH HHS / United States
R24 RR017386 / RR / NCRR NIH HHS / United States
P40 RR12546-03S1 / RR / NCRR NIH HHS / United States
5R24RR017386-02 / RR / NCRR NIH HHS / United States
R24 OD010998 / OD / NIH HHS / United States