TitleEnhanced sensitivity of lateral flow tests using a two-dimensional paper network format.
Publication TypeJournal Article
Year of Publication2011
AuthorsFu, E, Liang, T, Houghtaling, J, Ramachandran, S, Ramsey, SA, Lutz, B, Yager, P
JournalAnal Chem
Date Published2011 Oct 15
KeywordsChorionic Gonadotropin, Female, Humans, Immunoassay, Paper, Point-of-Care Systems, Pregnancy

Point-of-care diagnostic assays that are rapid, easy-to-use, and low-cost are needed for use in low-resource settings; the lateral flow test has become the standard bioassay format in such settings because it meets those criteria. However, for a number of analytes, conventional lateral flow tests lack the sensitivity needed to have clinical utility. To address this limitation, we are developing a paper network platform that extends the conventional lateral flow test to two dimensions. The two-dimensional structures allow incorporation of multistep processes for improved sensitivity, while still retaining the positive aspects of conventional lateral flow tests. Here we create an easy-to-use, signal-amplified immunoassay based on a modified commercial strip test for human chorionic gonadotropin, the hormone used to detect pregnancy, and demonstrate an improved limit of detection compared to a conventional lateral flow assay. These results highlight the potential of the paper network platform to enhance access to high-quality diagnostic capabilities in low-resource settings in the developed and developing worlds.

Alternate JournalAnal Chem
PubMed ID21936486
PubMed Central IDPMC3219509
Grant ListRC1 EB010593 / EB / NIBIB NIH HHS / United States
RC1 EB010593-02 / EB / NIBIB NIH HHS / United States
RC1EB010593 / EB / NIBIB NIH HHS / United States