Title | Enhanced sensitivity of lateral flow tests using a two-dimensional paper network format. |
Publication Type | Journal Article |
Year of Publication | 2011 |
Authors | Fu, E, Liang, T, Houghtaling, J, Ramachandran, S, Ramsey, SA, Lutz, B, Yager, P |
Journal | Anal Chem |
Volume | 83 |
Issue | 20 |
Pagination | 7941-6 |
Date Published | 2011 Oct 15 |
ISSN | 1520-6882 |
Keywords | Chorionic Gonadotropin, Female, Humans, Immunoassay, Paper, Point-of-Care Systems, Pregnancy |
Abstract | Point-of-care diagnostic assays that are rapid, easy-to-use, and low-cost are needed for use in low-resource settings; the lateral flow test has become the standard bioassay format in such settings because it meets those criteria. However, for a number of analytes, conventional lateral flow tests lack the sensitivity needed to have clinical utility. To address this limitation, we are developing a paper network platform that extends the conventional lateral flow test to two dimensions. The two-dimensional structures allow incorporation of multistep processes for improved sensitivity, while still retaining the positive aspects of conventional lateral flow tests. Here we create an easy-to-use, signal-amplified immunoassay based on a modified commercial strip test for human chorionic gonadotropin, the hormone used to detect pregnancy, and demonstrate an improved limit of detection compared to a conventional lateral flow assay. These results highlight the potential of the paper network platform to enhance access to high-quality diagnostic capabilities in low-resource settings in the developed and developing worlds. |
DOI | 10.1021/ac201950g |
Alternate Journal | Anal Chem |
PubMed ID | 21936486 |
PubMed Central ID | PMC3219509 |
Grant List | RC1 EB010593 / EB / NIBIB NIH HHS / United States RC1 EB010593-02 / EB / NIBIB NIH HHS / United States RC1EB010593 / EB / NIBIB NIH HHS / United States |