TitleExpression and activity of a novel cathelicidin from domestic cats.
Publication TypeJournal Article
Year of Publication2011
AuthorsLeonard, BC, Chu, H, Johns, JL, Gallo, RL, Moore, PF, Marks, SL, Bevins, CL
JournalPLoS One
Date Published2011 Apr 12
KeywordsAmino Acid Sequence, Animals, Anti-Infective Agents, Antimicrobial Cationic Peptides, Base Sequence, Cats, Circular Dichroism, DNA Primers, DNA, Complementary, Immunohistochemistry, Molecular Sequence Data, Polymerase Chain Reaction, Sequence Homology, Amino Acid

Cathelicidins are small cationic antimicrobial peptides found in many species including primates, mammals, marsupials, birds and even more primitive vertebrates, such as the hagfish. Some animals encode multiple cathelicidins in their genome, whereas others have only one. This report identifies and characterizes feline cathelicidin (feCath) as the sole cathelicidin in domestic cats (Felis catus). Expression of feCath is predominantly found in the bone marrow, with lower levels of expression in the gastrointestinal tract and skin. By immunocytochemistry, feCath localizes to the cytoplasm of neutrophils in feline peripheral blood. Structurally, the mature feCath sequence is most similar to a subgroup of cathelicidins that form linear α-helices. feCath possesses antimicrobial activity against E. coli D31, Salmonella enterica serovar Typhimurium (IR715), Listeria monocytogenes and Staphylococcus pseudintermedius (clinical isolate) similar to that of the human ortholog, LL-37. In contrast, feCath lacks the DNA binding activity seen with LL-37. Given its similarity in sequence, structure, tissue expression, and antimicrobial activity, the cathelicidin encoded by cats, feCath, belongs to the subgroup of linear cathelicidins found not only in humans, but also non-human primates, dogs, mice, and rats.

Alternate JournalPLoS One
PubMed ID21533281
PubMed Central IDPMC3075274
Grant ListT32 AI060555 / AI / NIAID NIH HHS / United States
T32 RR021312 / RR / NCRR NIH HHS / United States
T32RR021312 / RR / NCRR NIH HHS / United States
AI050843 / AI / NIAID NIH HHS / United States
T32AI60555 / AI / NIAID NIH HHS / United States
R37 AI032738 / AI / NIAID NIH HHS / United States
R01 AI050843 / AI / NIAID NIH HHS / United States