Title | Immunohistochemical characterization of intestinal neoplasia in zebrafish Danio rerio indicates epithelial origin. |
Publication Type | Journal Article |
Year of Publication | 2015 |
Authors | Paquette, CE, Kent, ML, Peterson, TS, Wang, R, Dashwood, RH, Löhr, CV |
Journal | Dis Aquat Organ |
Volume | 116 |
Issue | 3 |
Pagination | 191-7 |
Date Published | 2015 Oct 27 |
ISSN | 0177-5103 |
Keywords | Animals, Carcinoma, Fish Diseases, Immunohistochemistry, Intestinal Neoplasms, Zebrafish |
Abstract | Spontaneous neoplasia of the intestinal tract in sentinel and moribund zebrafish Danio rerio is common in some zebrafish facilities. We previously classified these tumors as adenocarcinoma, small-cell carcinoma, or carcinoma otherwise unspecified based on histomorphologic characteristics. Based on histological presentation, the primary differential diagnosis for the intestinal carcinomas was tumor of neuroendocrine cells (e.g. carcinoids). To further characterize the phenotype of the neoplastic cells, select tissue sections were stained with a panel of antibodies directed toward human epithelial (cytokeratin wide spectrum screening [WSS], AE1/AE3) or neuroendocrine (S100, chromogranin A) markers. We also investigated antibody specificity by Western blot analysis, using a human cell line and zebrafish tissues. Nine of the intestinal neoplasms (64%) stained for AE1/AE3; 7 (50%) also stained for WSS. None of the intestinal neoplastic cells stained for chromogranin A or S100. Endocrine cells of the pituitary gland and neurons and axons of peripheral nerves and ganglia stained for chromogranin A, whereas perineural and periaxonal cells of peripheral intestinal ganglia, and glial and ependymal cells of the brain stained for S100. Immunohistochemistry for cytokeratins confirmed the majority of intestinal neoplasms in this cohort of zebrafish as carcinomas. |
DOI | 10.3354/dao02924 |
Alternate Journal | Dis Aquat Organ |
PubMed ID | 26503773 |
PubMed Central ID | PMC4695397 |
Grant List | CA122959 / CA / NCI NIH HHS / United States P01 CA090890 / CA / NCI NIH HHS / United States P40 RR012546 / RR / NCRR NIH HHS / United States OD P40 RR012546 / OD / NIH HHS / United States CA090890 / CA / NCI NIH HHS / United States P01 HD022486 / HD / NICHD NIH HHS / United States P01HD22486 / HD / NICHD NIH HHS / United States P40 OD011021 / OD / NIH HHS / United States R01 CA122959 / CA / NCI NIH HHS / United States 2R24OD010998-11 / OD / NIH HHS / United States R24 OD010998 / OD / NIH HHS / United States P30 ES000210 / ES / NIEHS NIH HHS / United States |