TitleImmunohistochemical characterization of intestinal neoplasia in zebrafish Danio rerio indicates epithelial origin.
Publication TypeJournal Article
Year of Publication2015
AuthorsPaquette, CE, Kent, ML, Peterson, TS, Wang, R, Dashwood, RH, Löhr, CV
JournalDis Aquat Organ
Date Published2015 Oct 27
KeywordsAnimals, Carcinoma, Fish Diseases, Immunohistochemistry, Intestinal Neoplasms, Zebrafish

Spontaneous neoplasia of the intestinal tract in sentinel and moribund zebrafish Danio rerio is common in some zebrafish facilities. We previously classified these tumors as adenocarcinoma, small-cell carcinoma, or carcinoma otherwise unspecified based on histomorphologic characteristics. Based on histological presentation, the primary differential diagnosis for the intestinal carcinomas was tumor of neuroendocrine cells (e.g. carcinoids). To further characterize the phenotype of the neoplastic cells, select tissue sections were stained with a panel of antibodies directed toward human epithelial (cytokeratin wide spectrum screening [WSS], AE1/AE3) or neuroendocrine (S100, chromogranin A) markers. We also investigated antibody specificity by Western blot analysis, using a human cell line and zebrafish tissues. Nine of the intestinal neoplasms (64%) stained for AE1/AE3; 7 (50%) also stained for WSS. None of the intestinal neoplastic cells stained for chromogranin A or S100. Endocrine cells of the pituitary gland and neurons and axons of peripheral nerves and ganglia stained for chromogranin A, whereas perineural and periaxonal cells of peripheral intestinal ganglia, and glial and ependymal cells of the brain stained for S100. Immunohistochemistry for cytokeratins confirmed the majority of intestinal neoplasms in this cohort of zebrafish as carcinomas.

Alternate JournalDis Aquat Organ
PubMed ID26503773
PubMed Central IDPMC4695397
Grant ListCA122959 / CA / NCI NIH HHS / United States
P01 CA090890 / CA / NCI NIH HHS / United States
P40 RR012546 / RR / NCRR NIH HHS / United States
OD P40 RR012546 / OD / NIH HHS / United States
CA090890 / CA / NCI NIH HHS / United States
P01 HD022486 / HD / NICHD NIH HHS / United States
P01HD22486 / HD / NICHD NIH HHS / United States
P40 OD011021 / OD / NIH HHS / United States
R01 CA122959 / CA / NCI NIH HHS / United States
2R24OD010998-11 / OD / NIH HHS / United States
R24 OD010998 / OD / NIH HHS / United States
P30 ES000210 / ES / NIEHS NIH HHS / United States