TitleModeling of a competitive microfluidic heterogeneous immunoassay: sensitivity of the assay response to varying system parameters.
Publication TypeJournal Article
Year of Publication2009
AuthorsFu, E, Nelson, KE, Ramsey, SA, Foley, JO, Helton, K, Yager, P
JournalAnal Chem
Date Published2009 May 01
KeywordsAnimals, Binding, Competitive, Convection, Diffusion, Immunoassay, Mice, Microfluidic Analytical Techniques, Models, Chemical, Sensitivity and Specificity, Surface Properties

We present a fractional sensitivity analysis of a competitive microfluidic heterogeneous immunoassay for a small molecule analyte. A simple two-dimensional finite element model is used to determine the fractional sensitivity of the assay signal with respect to analyte concentration, flow rate, initial surface density of binding sites, and antibody concentration. The fractional sensitivity analysis can be used to identify (1) the system parameters for which it is most crucial to control or quantify the variability between assays and (2) operating ranges for these parameters that improve assay sensitivity (within the constraints of the experimental system). Experimental assay results for the drug phenytoin, obtained using surface plasmon resonance imaging, are shown to be consistent with the predictions of the model.

Alternate JournalAnal Chem
PubMed ID19361154
Grant ListU01 DE014971 / DE / NIDCR NIH HHS / United States
U01 DE14971-1 / DE / NIDCR NIH HHS / United States