TitleOptimization of assay conditions for leukotriene B4 synthesis by neutrophils or platelets isolated from peripheral blood of monogastric animals.
Publication TypeJournal Article
Year of Publication2005
AuthorsJha, S, Hall, JA, Cherian, G, Henry, LR, Schlipf, JW
JournalProstaglandins, leukotrienes, and essential fatty acids
Volume72
Issue6
Pagination423-30
Date Published2005 Jun
KeywordsTime Factors
Abstract

Neutrophils are involved in inflammation through leukotriene (LT) production. The predominant proinflammatory leukotriene released from neutrophils is LTB4, which serves as a biological marker of inflammation. The purpose of this study was to optimize the conditions ex vivo for LTB4 production by neutrophils from horses and dogs, and platelets from chickens. Optimal production of LTB4 was characterized by incubation time (2.5, 5, 10, 15 or 20 min), temperature (25 or 37 degrees C), and calcium ionophore A23187 concentration (0.1, 1, 10 or 20 microM). Incubation longer than 2.5 min did not increase production of LTB4 in chickens or horses; in dogs, incubation for 2.5 and 10 min resulted in the highest concentrations of LTB4 (P
DOI10.1016/j.plefa.2005.03.001