TitleRole of GerKB in germination and outgrowth of Clostridium perfringens spores.
Publication TypeJournal Article
Year of Publication2009
AuthorsParedes-Sabja, D, Setlow, P, Sarker, MR
JournalApplied and environmental microbiology
Date Published2009 Jun
KeywordsBacterial, Spores

Previous work indicated that Clostridium perfringens gerKA gerKC spores germinate significantly, suggesting that gerKB also has a role in C. perfringens spore germination. We now find that (i) gerKB was expressed only during sporulation, likely in the forespore; (ii) gerKB spores germinated like wild-type spores with nonnutrient germinants and with high concentrations of nutrients but more slowly with low nutrient concentrations; and (iii) gerKB spores had lower colony-forming efficiency and slower outgrowth than wild-type spores. These results suggest that GerKB plays an auxiliary role in spore germination under some conditions and is required for normal spore viability and outgrowth.