TitleSurface Plasmon Resonance-Based Concentration Determination Assay: Label-Free and Antibody-Free Quantification of Morpholinos.
Publication TypeJournal Article
Year of Publication2017
AuthorsBoutilier, J, Moulton, HM
JournalMethods Mol Biol
Volume1565
Pagination251-263
Date Published2017
ISSN1940-6029
KeywordsAnimals, Biosensing Techniques, Morpholinos, Surface Plasmon Resonance
Abstract

Surface plasmon resonance (SPR) is a physical process that allows label-free and real-time detection of biomolecular interactions. SPR provides a rapid and quantitative method for studying interactions of macromolecules such as proteins and nucleic acids. Antisense Morpholino oligomers are widely used to regulate gene expression and the US FDA has approved a Morpholino drug for treatment of Duchenne muscular dystrophy. Here, we describe an antibody-free, label-free, high-throughput, and walk-away SPR method for quantification of Morpholino compounds extracted from biological specimens. This provides a valuable way for determining pharmacokinetics and pharmacodynamics of Morpholino oligomers in biological matrices for research and therapeutic applications.

DOI10.1007/978-1-4939-6817-6_21
Alternate JournalMethods Mol Biol
PubMed ID28364249