TitleTetracycline resistance in Chlamydia suis mediated by genomic islands inserted into the chlamydial inv-like gene.
Publication TypeJournal Article
Year of Publication2004
AuthorsDugan, J, Rockey, DD, Jones, L, Andersen, AA
JournalAntimicrob Agents Chemother
Date Published2004 Oct
KeywordsAnimals, Bacterial Proteins, Blotting, Southern, Chlamydia, Chlorocebus aethiops, Chromosome Mapping, Cloning, Molecular, DNA Transposable Elements, Genes, Bacterial, Genome, Bacterial, Gram-Negative Bacteria, Microbial Sensitivity Tests, Plasmids, Repressor Proteins, Reverse Transcriptase Polymerase Chain Reaction, Swine, Tetracycline Resistance, Vero Cells

Many strains of Chlamydia suis, a pathogen of pigs, express a stable tetracycline resistance phenotype. We demonstrate that this resistance pattern is associated with a resistance gene, tet(C), in the chlamydial chromosome. Four related genomic islands were identified in seven tetracycline-resistant C. suis strains. All resistant isolates carry the structural gene tet(C) and the tetracycline repressor gene tetR(C). The islands share significant nucleotide sequence identity with resistance plasmids carried by a variety of different bacterial species. Three of the four tet(C) islands also carry a novel insertion sequence that is homologous to the IS605 family of insertion sequences. In each strain, the resistance gene and associated sequences are recombined into an identical position in a gene homologous to the inv gene of the yersiniae. These genomic islands represent the first examples of horizontally acquired DNA integrated into a natural isolate of chlamydiae or within any other obligate intracellular bacterium.

Alternate JournalAntimicrob Agents Chemother
PubMed ID15388463
PubMed Central IDPMC521927
Grant ListP30 ES00210 / ES / NIEHS NIH HHS / United States
R01 AI048769 / AI / NIAID NIH HHS / United States
AI42869 / AI / NIAID NIH HHS / United States
AI48769 / AI / NIAID NIH HHS / United States
P30 ES000210 / ES / NIEHS NIH HHS / United States