|Title||Therapeutic effects of paclitaxel-containing ultrasound contrast agents. |
|Publication Type||Journal Article |
|Year of Publication||2006 |
|Authors||Tartis, MShortencar, McCallan, J, Lum, AFH, LaBell, R, Stieger-Vanegas, SM, Matsunaga, TO, Ferrara, KW |
|Journal||Ultrasound in medicine & biology |
|Date Published||2006 Nov |
|Keywords||Cultured, Tumor Cells |
Drug delivery vehicles that combine ultrasonic and molecular targeting are shown to locally concentrate a drug in a region-of-interest. The drug delivery vehicles, referred to as acoustically active lipospheres (AALs), are microbubbles surrounded by a shell of oil and lipid. In a region limited to the focal area of ultrasound application, circulating AALs are deflected by radiation force to a vessel wall and can subsequently be fragmented. Ligands targeting the alphavbeta3 integrin are conjugated to the AAL shell and increase in vitro binding by 26.5-fold over nontargeted agents. Toxicity assays demonstrate that paclitaxel-containing AALs exert a greater antiproliferative effect after insonation than free paclitaxel at an equivalent concentration. Lastly, ultrasound and molecular targeting are combined to deliver a model drug to the endothelium and interstitium of chorioallantoic membrane vasculature in vivo.