Title | Ultrasound assessment of angiogenesis in a matrigel model in rats. |
Publication Type | Journal Article |
Year of Publication | 2006 |
Authors | Stieger-Vanegas, SM, Bloch, SH, Foreman, O, Wisner, ER, Ferrara, KW, Dayton, PA |
Journal | Ultrasound in medicine & biology |
Volume | 32 |
Issue | 5 |
Pagination | 673-81 |
Date Published | 2006 May |
Keywords | Doppler, Ultrasonography |
Abstract | Matrigel, a basement membrane extract, has been extensively used in in vivo angiogenesis. Contrast ultrasound imaging (CUI) of implanted Matrigel plugs with (+bFGF) and without basic fibroblast growth factor (-bFGF) was performed 7 and 14 d after implantation, followed by histologic analysis. Statistically significant differences between +bFGF and -bFGF plugs were apparent at d 7 in both plug size and contrast enhancement (both p < 0.05). Histopathology revealed differences in microvessel density (MVD) between +bFGF and -bFGF at d 7 and d 14. A significant correlation between MVD and both power Doppler contrast-enhanced area (r = 0.65, p < 0.05) and fraction of plug enhanced (r = 0.59, p < 0.05) was present. CUI of Matrigel plugs was shown to be a robust method for distinguishing between two different angiogenic states. Ultrasound measurements of blood flow in the plugs correlated with MVD, a histologic technique used to quantify tumor angiogenesis. |