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|Title||Use of a nonionic detergent (Triton WR 1339) in healthy cats to assess hepatic secretion of triglyceride.|
|Publication Type||Journal Article|
|Year of Publication||2000|
|Authors||Hall, JA, Gradin, JL, Andreasen, CB, Wander, RC|
|Journal||Am J Vet Res|
|Date Published||2000 Aug|
|Keywords||Animals, Area Under Curve, Caseins, Cats, Cholesterol, Erythrocyte Count, Female, Liver, Male, Oryza, Osmotic Fragility, Polyethylene Glycols, Surface-Active Agents, Triglycerides|
OBJECTIVE: To determine whether a nonionic detergent (Triton WR 1339) can be used in cats to assess hepatic secretion of triglyceride.
ANIMALS: 28 healthy cats.
PROCEDURE: Triton WR 1339 was administered IV according to the following schedule: 5, 50, 150, and 250 mg/kg of body weight. Control cats did not receive an injection or received 0.9% NaCl or PBS solutions at the same osmolarity and volume as the 250 mg/kg group. Blood samples were collected throughout the 48-hour period after administration for determination of triglyceride and cholesterol concentrations and for RBC morphology and osmotic fragility studies.
RESULTS: Administration of Triton WR 1339 at 150 and 250 mg/kg caused profound hypertriglyceridemia. Triglyceride concentrations increased in a curvilinear fashion for the first 2 hours and remained increased for approximately 24 hours. Area under the time-concentration curve for triglyceride at 5 hours differed significantly among groups. At 12 and 24 hours, cholesterol was significantly higher in cats receiving 250 mg/kg. The most dramatic changes in osmotic fragility and RBC morphology were in cats receiving 250 mg/kg; 1 of these cats developed severe icterus and died 5 days later. Feeding rice and casein before administering Triton WR 1339 at 150 mg/kg did not appear to affect the hypertriglyceridemia response.
CONCLUSIONS AND CLINICAL RELEVANCE: Triton WR 1339 can be administered IV to cats at a rate of 150 mg/kg to assess hepatic triglyceride secretion, although some cats may have increased RBC osmotic fragility. Higher dosages caused substantial adverse effects, whereas lower dosages did not alter plasma triglyceride concentration.
|Alternate Journal||Am J Vet Res|