Title | Use of a nonionic detergent (Triton WR 1339) in healthy cats to assess hepatic secretion of triglyceride. |
Publication Type | Journal Article |
Year of Publication | 2000 |
Authors | Hall, JA, Gradin, JL, Andreasen, CB, Wander, RC |
Journal | Am J Vet Res |
Volume | 61 |
Issue | 8 |
Pagination | 941-50 |
Date Published | 2000 Aug |
ISSN | 0002-9645 |
Keywords | Animals, Area Under Curve, Caseins, Cats, Cholesterol, Erythrocyte Count, Female, Liver, Male, Oryza, Osmotic Fragility, Polyethylene Glycols, Surface-Active Agents, Triglycerides |
Abstract | OBJECTIVE: To determine whether a nonionic detergent (Triton WR 1339) can be used in cats to assess hepatic secretion of triglyceride. ANIMALS: 28 healthy cats. PROCEDURE: Triton WR 1339 was administered IV according to the following schedule: 5, 50, 150, and 250 mg/kg of body weight. Control cats did not receive an injection or received 0.9% NaCl or PBS solutions at the same osmolarity and volume as the 250 mg/kg group. Blood samples were collected throughout the 48-hour period after administration for determination of triglyceride and cholesterol concentrations and for RBC morphology and osmotic fragility studies. RESULTS: Administration of Triton WR 1339 at 150 and 250 mg/kg caused profound hypertriglyceridemia. Triglyceride concentrations increased in a curvilinear fashion for the first 2 hours and remained increased for approximately 24 hours. Area under the time-concentration curve for triglyceride at 5 hours differed significantly among groups. At 12 and 24 hours, cholesterol was significantly higher in cats receiving 250 mg/kg. The most dramatic changes in osmotic fragility and RBC morphology were in cats receiving 250 mg/kg; 1 of these cats developed severe icterus and died 5 days later. Feeding rice and casein before administering Triton WR 1339 at 150 mg/kg did not appear to affect the hypertriglyceridemia response. CONCLUSIONS AND CLINICAL RELEVANCE: Triton WR 1339 can be administered IV to cats at a rate of 150 mg/kg to assess hepatic triglyceride secretion, although some cats may have increased RBC osmotic fragility. Higher dosages caused substantial adverse effects, whereas lower dosages did not alter plasma triglyceride concentration. |
DOI | 10.2460/ajvr.2000.61.941 |
Alternate Journal | Am J Vet Res |
PubMed ID | 10951988 |