Title | Analysis of pyroptosis in bacterial infection. |
Publication Type | Journal Article |
Year of Publication | 2013 |
Authors | Danelishvili, L, Bermudez, LE |
Journal | Methods Mol Biol |
Volume | 1004 |
Pagination | 67-73 |
Date Published | 2013 |
ISSN | 1940-6029 |
Keywords | Apoptosis, Bacterial Infections, Caspase 1, Cell Line, Cytokines, Cytological Techniques, Enzyme Assays, Humans, Interleukin-18, Interleukin-1beta, Macrophages, Mycobacterium tuberculosis |
Abstract | Eukaryotic cells undergo death by several different mechanisms: apoptosis, a cell death that prevents inflammatory response; necrosis, when the cell membrane lyses and all the intracellular content is spilled outside; and pyroptosis, a cell death that is accompanied by the release of inflammatory cytokines by the dying cells. Pyroptosis is designed to attract a nonspecific innate response to the site of infection or tumor. In this chapter, we describe the methods used to study pyroptosis in a mammalian cell. The model organism used is Mycobacterium tuberculosis, which suppresses pyroptosis by macrophages, and possibly in dendritic cells. |
DOI | 10.1007/978-1-62703-383-1_6 |
Alternate Journal | Methods Mol Biol |
PubMed ID | 23733570 |