TitlePhagocytosis of Loma salmonae (Microsporidia) spores in Atlantic salmon (Salmo salar), a resistant host, and chinook salmon (Oncorhynchus tshawytscha), a susceptible host.
Publication TypeJournal Article
Year of Publication2001
AuthorsShaw, RW, Kent, ML, Adamson, ML
JournalFish Shellfish Immunol
Date Published2001 Jan
KeywordsAnimals, Antigens, Protozoan, Macrophages, Microsporidia, Phagocytosis, Saccharomyces cerevisiae, Salmo salar, Salmon, Spores

The in vitro phagocytosis of Loma salmonae spores by macrophages of Atlantic salmon and two strains of chinook salmon were investigated. Opsonisation of L. salmonae with plasma factors increased uptake by head kidney macrophages. Macrophages of Atlantic salmon, which are resistant to the parasite, had a significantly higher phagocytic index (PI) than those of chinook salmon, a susceptible species. This may indicate a possible mechanism contributing to resistance in Atlantic salmon or that L. salmonae is able to evade or suppress initial binding by macrophages of chinook. Non-specific binding or lectinophagocytosis was also suggested by significantly higher PI of spores from EDTA treated plasma when compared with no plasma or heat treated plasma. In comparison, uptake of Baker's yeast Saccharomyces cerevisiae by phagocytes was not significantly different between fish species and strains for all treatments.

Alternate JournalFish Shellfish Immunol
PubMed ID11271605